Immunoblot and mass analyses of adiponectin produced in LH3 manipulated cell lines.

<p>(A) Recombinant adiponectin was produced in MEF cells and the medium containing the secreted adiponectin was collected for the analysis. The immunoblot analysis of recombinant adiponectin monomers from the concentrated medium of wild type, LH3^−/− knockout and LH mutant MEF cells indicates a clear size difference. (B) Schematic picture of the collagenous domain of mouse adiponectin shows the positions of posttranslational modifications of lysine and proline residues reported earlier. (C) Mass spectrometry identification of peptides and modifications from tryptic digests of mouse adiponectin produced in wild type, LH3^−/− knockout and LH mutant MEFs. Abbreviations: WT = wild type; KO = LH3^−/− knockout; MUT = LH mutant; Hyl = hydroxylysine; Gal = galactosyl; Glc = glucosyl.