The posterior to anterior myosin clearing is defective in <i>math-33(tm3561); usp-47(RNAi)</i>.

<p>(A) Time-lapse confocal images of embryos expressing NMY-2::GFP. The maximum projections of sections through the cortex of the embryo show the localization of NMY-2::GFP foci at three stages. Kymographs illustrate the pattern of myosin clearing and flow over time. About halfway through the kymographs (from top to bottom), large myosin foci transition into smaller myosin puncta. (B) The extent of myosin absence from the posterior (clearing) was measured as a proportion of the embryo length and the maximum level of clearing was recorded; each data point represents one embryo. (C) Confocal stacks of embryos immunostained for NMY-2 (green) and tubulin (red). Arrows indicate the location of the centrosome, red dotted circles mark the position of pronuclei, and the extent of myosin clearing is indicated by arrowheads. Scale bars represent 5 µm.