Transgenic markers are expressed by specific NSC niche cell types.

<p>(A) A schematic of the adult mouse forebrain in the coronal plane and cellular components of the neural stem cell (NSC) niche. Ependymal cells in <i>FoxJ1-Cre;R26^YFP/+</i> mice express YFP. NSCs, transit-amplifying progenitors (TAPs), and astrocytes in <i>Gli1^CreER/+;hGFAP-GFP;R26^tdTomato/+</i> mice express GFP/tdTomato, only tdTomato, and only GFP, respectively. Vascular endothelial cells in <i>Tie2-GFP</i> mice express GFP. LV: lateral ventricle, SVZ: subventricular zone. (B) A schema represents the induction of transgene in <i>Gli1^CreER/+;hGFAP-GFP;R26^tdTomato/+</i> mice by tamoxifen treatment. Three weeks after the treatment, SVZs were dissected, dissociated, and subjected to FACS or analyzed for the <i>in vivo</i> transgene expression. <i>FoxJ1-Cre;R26^YFP/+</i> or <i>Tie2-GFP</i> mice were also used for FACS or the <i>in vivo</i> transgene expression analysis. (C and D) A coronal section of the SVZ of <i>Gli1^CreER/+;hGFAP-GFP;R26^tdTomato/+</i> mouse. <i>GFP</i> expressing cells (green) are localized in the SVZ as well as in the striatum (St). <i>Gli1</i> lineage cells (tdTomato+, red) are located predominantly in the SVZ. Dashed line indicates the border between the lumen of the LV and the SVZ. (C) Immunofluorescent staining for GFAP (magenta) labels NSCs (GFP+/tdTomato+, arrow) and astrocytes (GFP+/tdTomato−, arrow head) in the striatum. TAPs (GFP−/tdTomato+, open arrow head) are not stained by GFAP. (D) Immunofluorescent staining for Sox2 (magenta) labels NSCs (GFP+/tdTomato+, arrow) and TAPs (GFP−/tdTomato+, open arrow head). (E) A coronal section of the SVZ of <i>FoxJ1-Cre;R26^YFP/+</i> mouse. YFP expressing <i>FoxJ1</i> lineage cells were stained by anti-GFP antibody to enhance fluorescence signal (green). S100β (red) labels GFP+ ependymal cells in the ventricular wall of the LV. (F) A coronal section of the SVZ of <i>Tie2-GFP</i> mouse. CD31 (red) labels GFP+ endothelial cells (green) in the SVZ. Scale bars: 20 µm (C,F), 10 µm (D,E). Nuclei were counterstained with Hoechst 33258 (blue).