Neutrophils are the predominant cell type that expresses IL-1β after <i>S. aureus</i> skin infection.

<p>pIL1-DsRed mice were infected intradermally with <i>S. aureus</i> and lesional skin specimens were collected at 4 and 24 hrs. Representative photomicrographs of sections labeled with anti-DsRed (IL-1β, red) and anti-MOMA2 (monocytes/macrophages, green) (A) or anti-7/4 (neutrophils, green) (C) and sections analyzed by confocal microscopy. Cells expressing both markers appear yellow (merge). High (left) and low (right) magnification images are shown (Scale bars = 50 µm). Dotted line = dermoepidermal junction. Quantification of co-localization of IL-1β-DsRed fluorescence with MOMA2⁺ monocytes/macrophages (B) or 7/4⁺ neutrophils (D) using the Manders' coefficient for a value range of 0 to 1 in which 0 = no pixels co-localize and 1 = all pixels co-localize. Data are representative from 4 mice per group.