hDPSCs surface antigens expression and <i>in vitro</i> multilineage differentiation ability.

<p>A: Cytofluorimetric analysis of Stro-1, c-Kit and CD34 expression in hDPSCs cultured in FCS-medium and in HS-medium. Dot plots reporting SSC vs fluorescence are shown. In the histograms, the net fluorescence value was calculated by linearizing the fluorescence value from the logarithmic scale and subtracting the linearized value of the unstained sample to the linearized value of the stained one. Data represent the mean±SD of three different experiments. * indicates values of paired <i>t</i>-test HS vs. FCS (*<i>p<</i>0.05). B: In the first line are shown double immunofluorescence images of hDPSCs/C₂C₁₂ co-culture stained by anti-hMit (green) and anti-myosin (red) Abs. DAPI staining is shown in blue. The second line shows oil red staining of hDPSCs differentiated for two weeks towards adipogenic lineage with HS or FCS supplemented medium. Cells were counterstained with Harris haematoxylin. Images, in third line represent anti-β3-Tubulin immunofluorescence labelling on hDPSCs differentiated in culture neurogenic media supplemented with FCS or HS. Bar 50 µm.