Northern analysis of arginine biosynthetic pathway in <i>S. aureus</i> JE2 and JE2 <i>ccpA::ermB</i>.
- Austin S. Nuxoll (116804)
- Steven M. Halouska (116806)
- Marat R. Sadykov (116808)
- Mark L. Hanke (116810)
- Kenneth W. Bayles (116812)
- Tammy Kielian (116814)
- Robert Powers (116816)
- Paul D. Fey (116818)
Publication date
November 2012
DOIAbstract
<p>JE2 and JE2 <i>ccpA::ermB</i> total RNA was isolated in mid-exponential phase of growth in CDM and CDM-R, respectively. DNA probes specific for <i>argJ</i>, <i>argB</i>, <i>argC</i>, <i>argD</i>, <i>argF</i>, <i>argG</i>, and <i>argH</i> were labeled with digoxygenin and detected using anti-digoxigenin-AP Fab fragments (Panel A). Panel B shows 16 s and 23 s rRNA depicting equal RNA loading.</p
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