Effect of natalizumab on CD4⁺ T-cell reactivity to myelin basic protein (MBP) <i>in vitro</i>.

<p>PBMCs were stained with CFSE and cultured for 7 days with MBP (30 µg/ml) and with natalizumab (NA; 25 µg/ml), IgG4 (25 µg/ml) or without further supplement for control (CON). Experiments were done with PBMCs from 4 healthy individuals. After staining for cell surface markers, we assessed (A) CD134 expression on CD26^HIGHCD3⁺CD4⁺ T-cells, proliferating in response to MBP and (B) proliferation of CD4⁺ T-cells by flow-cytometry. After stimulation with PMA/ionomycin for five hours, staining for surface-markers, permeabilization and staining for intracellular cytokines, the (C) IL-17 and (D) IFN-γ expression of MPB reactive CD4⁺ T-cells was assessed by flow-cytometry. Boxes represent interquartile range, median value indicated as a line, whiskers represent range, ^o = outliers, * = extremes. Statistics was by paired sample t-test.