IGF-1 Represses miRNA Expression.

<p>MCF-7 cells were grown in 5% charcoal stripped DMEM for 48 hours. Cells were treated for 2, 5, or 24 hours with 50 ng/ml IGF-1 or vehicle. Cells were collected for total RNA extraction and q PCR was performed for (A) total miR-let-7c, let-7g, miR-15b, miR-98, and miR-195 and (B) pre-let-7c, pre-let-7g, pre-mir-15b, pre-mir-98, pre-mir-195. Normalization was to U6 and vehicle treated cells designated as 1. Error bars represent SEM, n≤5. * Significantly different from IGF, p≤0.05.