Recombinant sea bass caspase-1 displays enzymatic activity.

<p>(<b>A</b>) Production and purification of recombinant sea bass caspase-1. W/o ind: non induced; ON ind: overnight induction; IB: inclusion bodies' fraction; Sol: soluble fraction; Refold: overnight refolding; Unb, Wash and Elution: non-bound, washed and eluted (1–4) fractions, respectively, of Ni-NTA chromatography purified refolded caspase-1. The positions of the p45, p24, p20 and p10 fragments are indicated. Numbers on the left indicate the position of the molecular weight markers in kDa. (<b>B</b>) Enzymatic activity of recombinant p24/p10 and p20/p10 sea bass caspase-1 heterodimers. p24/p10 and p20/p10 were standardized for 1 μg of p10 and were incubated with a specific fluorogenic substrate for caspase-1 in the presence or absence of the caspase-1 inhibitor Ac-YVAD-CHO. Hydrolysis of the substrate was followed by fluorimetry and results were expressed as relative fluorescent units (RFU).