Blue light-activated transcription regulation in zebrafish.

<p>A. Schematic of split Gal4 modules expressed in zebrafish together with a LUC reporter gene under control of a galactose-inducible promoter. B. Relative reporter activity (LUC/REN) in zebrafish expressing indicated genes (BD-CRY2N and AD-CIB1N). Zebrafish embryos were injected with the reporters and the effectors (BD-CRY2N and AD-CIB1N), kept in dark for 3 hours, then some irradiated with blue light (20 mmol m^–2 s^–1) for 2 hours (Blue) while some were kept in the dark (Dark). The relative LUC activities normalized to the REN activity are shown (LUC/REN). The experiment was repeated 3 times with pools of 30 embryos per condition each time. C. Relative reporter activity (LUC/REN) in zebrafish embryos expressing BD-CRY2N and AD-CIB1N in red or blue light. Embryos were injected with the reporters and the effectors (BD-CRY2N and AD-CIB1N), kept in dark for 3 hours, then some were irradiated with different fluence rate of blue light (42 to 120 20 mmol m^–2s^–1) for 2 hours (Blue) while others were irradiated with different fluence rates of red light (12 to 40 20 mmol m^–2s^–1) for 2 hours (Red). The relative LUC activities normalized to the REN activity are shown (LUC/REN, n = 3).