Bortezomib treatment caused permanent bone growth impairment in pair-fed animals. A)

<p>Schematic illustration of bortezomib treatment in C57B and NMRI mice. At start of treatment, day 0 (d0), mice received either 1 cycle of i.p. injected bortezomib (1 mg/kg) or vehicle on day 1, 4, 8 and 11, and were then sacrificed on day 13 (d13, 48 hrs post-treatment), day 56 (d56, 45 days post-treatment) or after 6 months (6 m). <b>B)</b> Animals were pair-fed and body weight was measured every day until day 20 after start of treatment, thereafter food was provided <i>ad lib.,</i> and body weight was measured once per week. From day 36 onwards body weight increase was suppressed in NMRI treated mice (*p<0.05 vs. vehicle). <b>C)</b> Permanent bone growth impairment was observed from day 13 until termination on day 56, with a significant decrease in femur length of bortezomib treated mice vs. vehicle of the same strain (**p<0.01 on d13 and ***p<0.001 on d56). The vertical dashed lines in panels B and C indicate the last injection of bortezomib. Data represent mean±SEM.