Changes in the levels of sphingolipid species during acetic acid-induced apoptosis.

<p>Relative levels of dihydrosphingosine (DHS), dihydrosphingosine-1-phosphate (DHS-1-P), phytosphingosine (PHS), phytosphingosine-1-phosphate (PHS-1-P), phytoceramides (Phyto-C₁₆-Cer and Phyto-C₁₈-Cer) and α-hydroxylated phytoceramide (α-OH-Phyto-C₂₀-Cer) in <i>S. cerevisiae</i> CG379 (wild-type), <i>lag1</i>Δ and <i>isc1</i>Δ strains after exposure to 180 mM acetic acid, pH 3.0, for 200 min are shown. Values are mean ± SD of three independent experiments. * P<0.05, ** P<0.01, One-way ANOVA and Tukey Test. Levels of α-OH-Phyto-C₂₀-Cer in CG379 reproducibly displayed a high increase after exposure to acetic acid in all experiments, though relative values varied considerably.