Circadian rhythm in <i>Gclm</i> expression persists in constant darkness.

<p>(A) <i>tim</i> and (B) <i>Gclm</i> mRNA expression show a circadian rhythm in heads of CS flies on the second day of constant darkness. An asterisk indicates a significant difference in the expression level between the trough of each gene and the peak (p<0.05). (C) No significant rhythm was detected in <i>Gclc</i> mRNA levels in wild type flies. Data represents average values obtained from 3 independent bio-replicates (± SEM) and normalized to ZT 0. Significance was calculated by a 1-way ANOVA and Bonferroni's multiple comparison post-tests. CT = Circadian Time. Shaded horizontal bars indicate subjective day.