Annexin A2 translocates into the nucleus in response to genotoxic agents.

<p>TIME cells were not treated (NT) or treated with: (A) 1.5 J/m² UV-A (365 nm) (UV), 10 Gy Gamma-radiation (IR), 8.5 µM etoposide (etp) or 20 µM chromium VI (Cr6⁺) for 2 hours; (B) with the indicated doses of Gamma-radiation (IR) for 2 hours; (C) with 10 Gy of IR for the times indicated; (D) with the indicated doses of UV-A radiation for 2 hours; (E) with 1.5 J/m² UV-A (365 nm) for the times indicated; (F) with 20 µM chromium VI (Cr⁶⁺) for the times indicated; (G) with 10 Gy of IR in the absence or presence of Wheat Germ Aglutinin (WGA) for the times indicated. (A–G) Nuclear and Non Nuclear fractions were prepared and identical ratios, representing the same percentage of each subcellular fraction were subjected to SDS-PAGE followed by western blotting with the antibodies indicated. Protein markers for the sub-cellular fractions include: nucleolin (nucleus); lamin A/C (nuclear membrane); tubulin (cytoplasm); CD146 (plasma membrane). (H) TIME cells were either not treated (NT) or treated with 10 Gy IR for 1 hour. Cells were subjected to immunocytochemistry analysis with the antibodies indicated and visualized by confocal microscopy. Scale bar is 20 µM.