Confocal photomicrographs of ESMV-treated and control Müller cells immunostained for markers of various retinal lineages.

<p>In A–L, cells were double stained with Gad67 (amacrine & horizontal cells; green) or NeuN (amacrine and ganglion cells; green) and the marker of Müller cells, Glutamine Synthetase (red). (A–C): Gad67-stained ESMV-treated Müller cells. (D–F) Gad67-stained control Müller cells. (G–I) NeuN-stained ESMV-treated Müller cells. (J–L) NeuN-stained control Müller cells. The third panel of each row shows the merged first two images. (M) ESMV-treated and (N) control Müller cells, stained for Brn3a (green), a marker of retinal ganglion cells. (O) ESMV-treated and (P) control Müller cells stained for Syntaxin 1a (green), a marker of amacrine cells. (Q) ESMV-treated and (R) control Müller cells stained for rhodopsin (green), a marker of rod photoreceptors. Cell nuclei were labeled with 4′6-diamidino-2-phenylindole (DAPI, blue). Scale bar 10 μm for all panels. Images show z-axis projections of 15×1 μm in all channels.