tbx5 over-expression causes eye, cardiac and fin defects.

<p>A, phenotypes generated by increasing doses of <i>tbx5a</i> mRNA. The percentage of embryos with the indicated defects was averaged across multiple independent experiments. The total number of embryos analyzed was as follows: mRNA-Tbx5a (35 pg) n = 48; mRNA-Tbx5a (100 pg) n = 199; mRNA-Tbx5a (200 pg) n = 131;. B, qRT-PCR analysis of <i>miR-218</i>a relative expression in 24 and 34 hpf embryos injected with 100 pg of <i>tbx5a</i> mRNA compared with embryos injected with 100 pg of GFP mRNA. C, phase-contrast and confocal images of representative transgenic <i>Tg(cmlc2:eGFP)</i> embryos at 72 hpf showing eye, heart and fin morphological defects induced by the injection of 100 pg (b,b′–c-c′) or of 200 pg (d,d′) of <i>tbx5a</i> mRNA. Arrowheads indicate eye alteration, arrows show fin absence. Labels: A, atrium, V, ventricle. Dotted lines encircle ventricle (white) and atrium (red). Scale bars: black 100 µm, red 25 µm.