MSCV promoter-mediated expression of GFP in various types of cultured cells.

<p>(A) Lentiviral vectors express enhanced green fluorescent protein (GFP) under the control of the MSCV promoter. To remove TAT-dependent transcription, the U3 region of the LTR was deleted. The Δgag (deleted gag sequence) was included to enhance packaging efficiency. For improved viral-mediated gene transfer, the central polypurine tract (cPPT) and central termination sequence (CTS) were added in Δpol (deleted pol sequence). RRE; Rev response element. (B) Expression of GFP in mouse embryos. (C) Expression of GFP in human embryonic kidney (HEK) 293T cells. (D) Cerebellar neuronal culture infected with lentiviral vectors at 1 day in vitro (DIV) and immunolabeled for neuron-specific nuclear protein (NeuN) at DIV 15. In addition to Purkinje cells (arrows), GFP was expressed in granule neurons (arrowhead, inset) and in glia (arrow, inset). Scale bars, 20 µm (B), 25 µm (C), 50 µm (D).