Quantitative analysis of liver lipid level and composition with biochemical assay and multimodal CARS microscopy.

<p>(<b>A</b>) Triglyceride concentration in milligram per gram liver tissue measured with a commercial triglyceride quantitation kit. (<b>B</b>) Liver lipid level determined with CARS signal intensity and normalized to 1 for wildtype mice and respectively for other animal groups. Error bars in <b>A</b> and <b>B</b> are standard deviations across 9 mice analyzed per animal group. (<b>C</b>) Representative Raman signatures of single liver lipid droplets of wildtype (light green), UPase-TG (blue), and wildtype+fenofibrate mice (red). (<b>D</b>) Lipid unsaturation (I₁₆₆₀/I₁₄₄₅) of liver lipid droplets as a function of animal group. Error bars are standard deviations across 60 lipid droplets analyzed per animal group (9 mice per group).