Bcl-xL Expression is Regulated by IL-6 Cytokines and SOCS3.

<p><b>A</b>–<b>B,</b> Primary neurons (A) were treated with OSM (10 ng/ml) for the times indicated, or SH-SY5Y cells (B) were treated with IL-6 (10 ng/ml) plus sIL-6R (25 ng/ml) or OSM (10 ng/ml) for the times indicated. Protein lysates were prepared and subjected to immunoblot analyses with antibodies against Bcl-xL and GAPDH. The densitometric ratios of Bcl-xL versus GAPDH were calculated. Graph represents the mean ± SEM of triplicate cultures in three separate experiments. *<i>p</i><0.001, and **p<0.01 compared to control. <b>C</b>. Co-IP of Bax with Bcl-xL. Lysates from each sample were subjected to immunoprecipitation (IP) using antibody specific for Bax. IP samples were probed with antibody to Bcl-xL to demonstrate a specific interation between Bcl-xL and Bax. The densitometric ratios of co-immunoprecipitated Bcl-xL to immunoprecipitated Bax were calculated. Graph represents the mean ± SEM of triplicate cultures in three separate experiments. *<i>p</i><0.001, **p<0.01, and ***p<0.05 compared to control. <b>D</b>, SH-SY5Y cells were infected with pGipz or Lenti-shSOCS3. After infection, IL-6 plus sIL-6R was added for the times indicated, and cell lysates immunoblotted as described. The densitometric ratios of Bcl-xL versus GAPDH were calculated. Graph represents the mean ± SEM of triplicate cultures in three separate experiments. *<i>p</i><0.001, and **p<0.01 compared to pGipz-infected cultures; and &p<0.01 compared to IL-6 plus IL-6R treatment of pGipz-infected cultures.