Target validation using luciferase reporter assays.
- Ling Lian (114953)
- Lujiang Qu (114956)
- Yanmei Chen (114960)
- Susan J. Lamont (114962)
- Ning Yang (114965)
Publication date
November 2012
DOIAbstract
<p>A. Interaction validation for gga-miR-181a and MYBL1. B. Interaction validation for gga-miR-181a and IGF2BP3. C. Interaction validation for gga-miR-26a and EIF3A. D. Interaction validation for gga-miR-221 and BCL11B. 293T cells were co-transfected with pmiR-RB-REPOR-UTR, or pmiR-RB-REPOR-mutated UTR and miRNA mimic, or negative control. Gene-wtUTR: wild type 3′UTR of predicted target gene. Gene-mutUTR: Mutated 3′UTR of predicted target gene. NC: Negative control.</p
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