Detection of vimentin <i>in vivo</i> in Dcn^−/− and wild-type mouse skin and <i>in vitro</i>.

<p>(A) Western blot for vimentin (57 kDa) with two different skin extracts of adult wild-type and Dcn^−/− mice (upper panel). Coomassie gel was used as loading control and shows the corresponding protein extracts (lower panel). (B) Quantification of Western blots, where the vimentin signal was normalized to the Coomassie gel staining (n = 4; **, p<0.01). (C) Dcn^−/− fibroblasts were cultured for 3, 6 and 14 days in the presence of ascorbate-2-phosphate and treated with decorin or decorin core (core) and the respective controls. Protein extracts were immunoblotted with antibodies to detect vimentin. Coomassie gel was used as loading control (lower panel, exemplary). (D) Quantification of vimentin protein expression at day 3, 6 and 14. Western blot signals were normalized to Coomassie staining. Data represent 3 independent experiments and are expressed as mean ± SD (*, p<0.05).