Inhibition of GroES-E321K refolding activity by mHsp10.

<p>A binary complex of E321K and HCl-denaturated MDH was pre-incubated for 30 min in the presence of increasing concentrations (from 0 to 20 µM) of mHsp10 and 2 mM ATP before adding 20 µM GroES. MDH activity was measured 1 hour following the addition of GroES. % inhibition = 100*[(A_o–A_i)/A_o]. A_o represents the activity level in the absence of mHsp10, and A_i represents the activity level at each mHsp10 concentration.