Proliferation of LSK cells during marrow regeneration.

<p>(A) Sub-lethally irradiated mice were maintained (without transplantation) for different time points. Mice were sacrificed and LSK cells were determined by flowcytometry. Bar diagram shows proliferation kinetics of host BM-LSK cells (n = 6, each time point); (B) Each sub-lethally irradiated mouse received 10×10⁶ crude BM cells of CD45.2 mouse. Mice were sacrificed and LSK cells were determined by flowcytometry. Bar diagram shows proliferation kinetics of host and donor LSK cells (n = 6, each time point); (C) Each sub-lethally irradiated mouse received 3×10⁴ CD45.2LSK cells. Mice were sacrificed and LSK cells were determined by flowcytometry. Bar diagram shows proliferation kinetics of host and donor LSK cells (n = 3, each time point); (D, E) Competitive marrow repopulation assay. Donor hematopoietic (CD45.2⁺) cells of 10 and 15 days of transplantation were isolated from primary recipient. Four different doses of above sorted cells (10×10³, 30×10³, 100×10³ and 300×10³) were transplanted in 4 groups of mice, each consisting of 4. After 1 month of transplantation, BM cells were isolated from secondary recipient and analyzed for donor-derived LSK cells. Proportional increase in the recovery of donor LSK with transplantation dose are shown in E.