Principal component analysis of CHCs detected by GC-MS in <i>D. melanogaster</i> females fed four different diets.

<p>Three PCs were retained that explained 56% total variance, with PC1, PC2, and PC3 explaining 22%, 18%, and 16% of variance after Varimax rotation. PC scores are plotted in 3D space (A), and orthogonal best fit lines for PC scores serve as axes of cones with apexes pointing in the direction of increasing fly age. The numbers designate fly ages: 1 = 7d, 2 = 23d, 3 = 49d, 4 = 65d. Colors indicate food treatments: Grey = S5Y5, Red = S5Y20, Blue = S20Y5, Green = S20Y20. The arrows on PC1-PC2 plane represent the projections of each cone’s axis. PC loadings are shown in the table (B) with shading reflecting the strength of each CHC’s load on each PC (as rendered by JMP). For statistical treatment of individual CHCs, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0049799#pone.0049799.s002" target="_blank">Fig. S2</a>.