The D1 protein of PSII is protected from damage caused by oxidative stress and severe dehydration.

<p>(<b>A</b>) Damage by the H₂O₂ treatments (H₂O₂) compared to control conditions (C). (<b>B</b>) Protection from damage by the dehydration treatments (DT). The DT samples were analyzed immediately after the dehydration treatment (0 h), and following rehydration for 16 h (16 h). Thylakoid protein samples from the indicated transgenic lines (T) were compared with sibling non-transgenic (NT) lines. Protein amounts loaded per lane in order to obtain similar initial amounts of the D1 protein: NT₁ (40 μg), T₁ (10 μg), NT₂ (20 μg), T₂ (10 μg), NT₃ (20 μg), and T₃ (10 μg). Immunoblot detection was performed with anti-D1 (DE-loop) antibodies at 1/2,000 (A) or 1/6,000 dilution (B). To the right of each panel, a thin line marks the position of the major D1 band; slightly above, a shaded square marks the retarded mobility D1 band observed after the stress treatments. Asterisks above and below the major D1 bands respectively indicate the cross-linked and degraded D1 protein bands mentioned in the text. Molecular mass markers (kD) are indicated to the left.