The <i>C. albicans</i> Set3C is a coding sequence histone deacetylase.

<p>(A) Architecture of the <i>S. cerevisiae</i> Set3C. The subunits among which physical interaction was confirmed in <i>C. albicans</i> are colored green (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003118#pgen.1003118.s001" target="_blank">Figure S1B</a>). (B) Physical interaction of Set3 and Hos2. Set3-3HA was immunoprecipitated from whole cell extracts and the interaction was probed by immunoblot detection of a Hos2-9myc allele. (C) Read density profiles of one replicate of a Set3-9myc and an untagged control ChIP-Seq experiment. Genes were divided into binding targets (“targets”) and non-targets (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003118#s4" target="_blank">Materials and Methods</a>). Transcription start site (TSS) denotes the start codon and Transcription termination site (TTS) denotes the stop codon. The read density values between the TSS and TTS were calculated to a percentage scale, and 500 bases upstream of the TSS and downstream of the TTS were included. On the bottom panel only genes with a coding region longer than 1 kilobase were included. (D) Definition of CaSet3C refined target gene set. Each dot corresponds to one ORF. Binding targets of RNAPII transcribed genes are defined as having an at least 2-fold enrichment on one axis and an at least 1.5-fold on the other axis (blue box). Target tRNA loci are defined as having an at least 1.5-fold enrichment on both axes. The complete dataset is found in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003118#pgen.1003118.s011" target="_blank">Tables S4</a> and <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003118#pgen.1003118.s012" target="_blank">S5</a>. “<i>r</i>” denotes a Pearson's correlation coefficient. (E) The Set3C functions as histone deacetylase <i>in vivo</i>. Top panel: validation of Set3 and Hos2 binding using the indicated probes around the <i>PFK1</i> and <i>tR(CCG)1</i> loci by qPCR. Values are normalized to a fragment of the <i>ADE2</i> locus. Bottom panel: ChIP experiments were performed with antibodies against acetylated histone H4 and the C-terminus of histone H3. The qPCR values at the probe positions were normalized to a fragment of the telomere of Chromosome 7. The ratio of the signal of the acetylated H4 ChIP and H3 ChIP is shown on the y-axis. Data are shown as mean+SD of three independent experiments. Statistical significance was determined by two-tailed t-test relative to the control values. *P<0.05, **P<0.01, ***P<0.001.