The role of arginine residues within the dynamin-1 PRD in pacsin-1

<p>'<b>s membrane deformation potential.</b> A. Membrane deformation of Folch liposomes. The positions of Arg-to-Ala mutations (GST-PRD^ArgKO1, GST-PRD^ArgKO2 and GST-PRD^ArgKO3) in the mouse dynamin-1 PRD protein sequence are shown (top panel). Negative-stain EM images are shown after incubation of liposomes with the indicated protein complexes. B. Liposome co-pelleting assay. Liposome binding assays were carried out as described in Fig. 3. Error bars represent standard deviations of a minimum of 3 independent experiments.