Activation of full-length endophilin-A1 by GST-PRD.

<p>A. Negative-stain EM with Folch liposomes. Assays with endophilin-A1 (full-length or N-BAR domain; 10 µM) were carried out as described before. The inset shows a zoomed-in view of the red box area of the image, with scale bar = 100 nm. B. Statistical analysis of vesicle size distribution. Diameters of vesicles produced by endophilin in the presence of GST-PRD were quantified from electron micrographs taken from three independent experiments. C. Liposome co-pelleting assay with Folch liposomes. Liposome binding assays were carried out as described in Fig. 3. The horizontal, dashed lines indicate the lipid-bound fraction of the isolated endophilin-A1 N-BAR (expressed as His₆-SUMO-fusion protein) domain and isolated full-length endophilin-A1 under similar conditions. Error bars represent standard deviations of a minimum of 3 independent experiments.