blot analysis of PrP^C.

<p>Brain or spinal cord tissue was homogenized and analysed by Western blot as described in <i>Material & Methods</i> with various anti-PrP antibodies. Hooded Seal, one sample lanes 1–6; domestic dog, one sample lanes 7–8; domestic cat, two samples, 9–10 and 11–13; Brown bear, three samples 14–15, 16–17, 18–19. Untreated PrP^C: lanes 1,3,5,7,9,11,14,16,18. Deglycosylated PrP^C (+): lanes 2,4,6,8,10,12,13,15,17,19. Detection using antibodies with epitopes N-terminal to α-cleavage site: SAF32 (lanes 1–2) or F11 (lane 12), with epitope in α-cleavage site: 3F4 (lanes 3–4), with epitopes C-terminal to α-cleavage site: Bar224 (lanes 5–10, 14–19), 6H4 (lane 11) or BC6 (lane 13).