Characterization of clonally derived bone marrow MSC progenitors.

<p>(A) Cellular cloning of conditionally immortalized MSCs was performed by limiting dilutions, and the resulted single-cell derived subpopulations were cultured in 96-well plates and screened for the potential for osteogenic and adipogenic differentiation. (B) Differentiation assay revealed the bipotential clones capable of osteo- and adipogenesis “OA”, and monopotential osteo- and adipogenic clones, “O” and “A”. (C) Summary of screening of two independent MSC lines. (D) Flow cytometry results of staining of clonally derived MSC progenitors with OA, O and A potential, for the differentially expressed markers CD200, SSEA4 and CD140a. The percent of cells in the positive gate is indicated on the histogram. (E) Comparison of levels of differentially expressed markers CD200, SSEA4 and CD140a shown by Mean Fluorescence Intensity of flow cytometry measurement, between OA, O and A progenitors. 4 clones of each type were used for experiment, statistical significance is indicated.