Lumican inhibits MMP-14 expression and activity in MSC.

<p>(A): Lumican effect on MMP-14 expression in MSC and EPC analyzed by Western blotting using a mouse anti-MMP-14 monoclonal antibody directed against the catalytic domain of MMP-14 and probing with anti-β-actin. Semi-confluent MSC and EPC were seeded on plastic or in presence of lumican coating as described in Materials and Methods section. Inactive and active MMP-14 forms were detected in positive control cell lysates of fibrosarcoma cell line (HT-1080). Active MMP-14 was not detected in negative control cell lysates of HeLa cells. (B): MMP activity was measured using a SensoLyte® 520 MMP-14 Assay Kit as described in the Materials and Methods section. The activity of MMP-14 of cell lysates of MSC and EPC seeded on plastic or lumican coating (30 µg/cm²) was measured from four different donors in two independent experiments. Data presented as mean ± S.D. **<i>p</i><0.01. (C): MMP-2 and MMP-9 expression and activity in MSC and EPC. MMP-2 and MMP-9 expression and activity in cell lysates (left panel) and conditioned cell media (right panel) were analyzed by zymography.