Similarity in amino acid sequences between rat and human biomarkers and predicted protease activities.

<p>a) Amino acid sequence orthology between the 50 most significant human biomarkers for CKD and CVD and the defined 180 rodent markers for diabetes associated complications. To examine the orthology between human and rat markers we applied three criteria: first, we looked for identical fragments (both cleavage sites identical in human and rat); secondly, we looked for fragments with one identical cleavage site and third, we looked for peptides from the same protein area with a minimum overlap in two amino acids. b) Predicted protease activities related to rat and human COL1A1 biomarker fragments. The relative number of specific cleavage sites for MMPs, ADAMTS5, CTSK and F2 and direction of amplitude regulation for COL1A1 markers in rat and human models are given.