Axon branching on various substrates.

<p>(<b>A</b>) Immunofluorescence labeling shows a neuron (identified by β3 tubulin staining) with one branched axon. (<b>B</b>) Quantification of branches per neuron on glass coated with various substrate molecules. (<b>C</b>) Quantification of branches per neuron on various ALCAM nanopatterns. Error bars represent SEM; for statistical analyses, two-way ANOVA followed by post-hoc comparisons using two-tailed Student's t test with Bonferroni-Holm corrections were performed (***P<0.001, **P<0.01, *P<0.05).