EGF+E2 treatment leads to Erk1/2 MAPKs and Akt hyperactivation in p130Tg organoids.

<p>(A) Western blot analysis of phospho-Erk1/2 MAPKs in wt or p130Tg organoids stimulated with EGF and EGF+E2 at the indicated times. Vinculin and total Erk1/2 MAPKs blots are provided as loading controls. Blots are representative of five independent experiments. (B) Quantification analysis of Erk1/2 MAPKs activation following stimulation for 5, 30 minutes and 1 hour. Data are represented as the mean of phospho-Erk1/2 MAPK levels/total Erk1/2 MAPK ± standard deviation (SD) of five independent experiments (*p<0.05). (C) Western blot analysis of phospho-Akt in wt or p130Tg organoids stimulated as in (A). Total Akt blots are provided as loading controls. Blots are representative of three independent experiments. (D) Quantification analysis of Akt activation following stimulation as in (A). Data are represented as the mean of phospho-Akt levels/total Akt ± standard deviation (SD) of three independent experiments (*p<0.05).