Ca²⁺ imaging of cholinergic DA motoneurons in freely moving <i>C. elegans</i>.

<p> <b>A</b>, Confocal images of L1 larvae expressing G-CaMP6 (<i>jqEx97</i>) or G-CaMP3 (<i>jqEx216</i>) in the DA motoneurons. In both transgenic strains, DsRed-Express-1 is co-expressed in the DA motoneurons. TL, transmitted-light image. Arrows indicate the DA7 motoneuron analyzed in <b>B</b>. <b>B,</b> Representative spontaneous fluorescence responses (Δ<i>R</i>/<i>R</i>) of G-CaMPs from DA7 cholinergic neurons in transgenic worms during locomotion. <b>C</b>, Mean peak responses (Δ<i>R</i>/<i>R</i>). Error bars, s.e.m. (<i>n</i> = 10 each from 4 worms, *<i>P</i> = 0.0020, Student’s <i>t</i>-test). Movies of the recordings are available as supplementary information (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051286#pone.0051286.s001" target="_blank">Movies S1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051286#pone.0051286.s002" target="_blank">S2</a>).