Growth on uric acid and glycine (A) and corresponding gene expression at different time points (B).

<p>(<b>A</b>) Growth of cells was measured over 12 h in medium containing different concentrations of uric acid (UA) with (dashed line) or without (solid line) glycine (Gly). Non-adapted cells were shifted directly from uric acid medium to uric acid with glycine whereas adapted cells were grown three times in 12 mM uric acid medium with 100 mM glycine prior to transfer and measurement. (<b>B</b>) Dependent on the addition of glycine a semi-quantitative analysis of transcription levels of genes involved in the conversion of glycine to acetate was performed. Shown are the results for adapted cells grown in 3 mM and 12 mM uric acid medium or in the same media supplemented with 100 mM glycine. Samples were taken after 1 h, 3 h, 5 h, 7 h and 9 h. DNA as template in the RT-PCR reaction served as positive control (P) and water as negative control (N). Abbreviations: <i>gcvPB</i>, glycine dehydrogenase beta subunit (Curi_c00770); <i>glyA</i>, serine hydroxymethyltransferase (Curi_c17240); <i>ackA,</i> acetate kinase (Curi_c16390); <i>por1/por2</i>, pyruvate:ferredoxin oxidoreductase (Curi_c20330/Curi_c21330); <i>fdhA</i>/<i>fdhB</i>, formate dehydrogenase subunits A; (Curi_c16670, Curi_c16640); <i>fdhF1/fdhF2</i>, formate dehydrogenase H (Curi_c29370/Curi_c29380); <i>rpoA</i>, DNA-directed RNA polymerase alpha subunit (Curi_c22350, reference).