Effect of over-expression of Bcl-xL and cFLIP in E2F1-induced apoptosis.

<p>Stable ER-E2F1 PC12 cells were transfected or not with Bcl-xL (A) or cFLIP (B) expression plasmids and treated (+) or not treated (−) with OHT. At 48 hours, apoptotic cells were identified by TUNEL and counted against 100 nuclei identified by Hoescht staining in randomly taken photographs. Results are presented as Mean ± SEM, for n = 3. Statistically significant differences were obtained comparing with cells untransfected. Student’s <i>t</i>-test value of **p<0,01 was considered statistically significant. Expression of the transfected proteins were measured by Western Blot analysis and shown on the right of the figures. HA antibody was used for Bcl-xL detection and FLAG for FLIP. Arrow indicates FLIP position and asterisk a non specific protein.