Expansion of iNKT cells in draining lymph nodes of α-GalCer-treated mice.

<p>Mice were immunized with GPI peptide and then treated with either DMSO or α-GalCer and euthanized on day 6 or 10. The dLNs were obtained and examined for the presence of iNKT cells by FCM (Data are mean±SD. n = 4–5. Data are representative results of two experiments). (<b>A</b>) iNKT cells were detected as TCRβ and α-GalCer-loaded CD1d-tetramer double positive cells. (<b>B</b>) Proportion of iNKT cells among TCRβ+ αβT cells, and (<b>C</b>) absolute number of iNKT cells in dLNs of naive or immunized mice. *p<0.05 by Man-Whitney analysis.