MTG family members depend on Kaiso to repress the human <i>MMP-7</i> promoter.

<p>A. The composition of the full-length <i>MMP-7</i> reporter (HMAT-2.3). The HMAT-2.3 reporter contains two Kaiso and two TCF binding sites followed by a luciferase gene. B. HMAT-2.3 artificial promoter assays in HCT116 cells upon titration of MTG family members (0 ng, 200 ng, 400 ng, 800 ng). The graph shows the fold-change in luciferase activity relative to the control reporter, pGL4-TK hRLUC, after transfection of expression plasmids encoding MTG family members, MTG8, MTG16, and MTGR1. The error bars represent the standard error of the mean for 3 replicate experiments performed in triplicate. C. HMAT-2.3 promoter assay utilizing MTG16 deletion constructs in which the indicated nervy homology region is removed. Error bars represent the standard deviation of triplicate samples. D. HMAT-2.3 artificial promoter assay after knockdown of Kaiso and the transfection of 500 ng of MTG16. Error bars represent the standard deviation of triplicate samples. *<i>P</i><0.05, ***<i>P</i><0.001.