Apoptotic function of E2F1 is correlated with its ability to induce ROS accumulation.

<p>(A) Indicated SH-SY5Y and SK-N-JD neuroblastoma ER-E2F1 stable cell lines were treated (+) or not treated (−) with OHT, in the presence (+) or absence (−) of serum for 4 hours. Cell extracts were collected and lysed, and caspase-3 activities (left panels) and ROS levels (right panels) were analysed as described in Material and Methods. (B) Indicated SH-SY5Y and SK-N-JD neuroblastoma ER-E2F1 stable cell lines were treated (OHT) or not treated (control) with OHT for 4 hours. Cells were immunostained for E2F1 protein (red) and DAPI (blue). (C) Indicated SH-SY5Y and SK-N-JD neuroblastoma ER-E2F1 stable cell lines were transfected for 16 hours with a E2F luciferase reported construct and treated with (+) or without (−) OHT. Luciferase was measured and normalized for <i>Renilla</i> luciferase readings in the same extracts and the values were indicated. Results are presented as Mean ± SEM, for n = 4. In all figures, Student’s <i>t</i>-test values of *p<0,05, **p<0,01 and ***p<0,0001 were considered statistically significant.