Histological analyses of control and transgenic seeds.

<p><b>(A).</b> Starch granules were seen in control seeds at 7 DAP. <b>(B, C).</b> m- and s-seeds at 7 DAP without starch granules. <b>(D–F).</b> A higher magnification of the OV at 7 DAP in the control seeds (D), m-seeds (E) and s-seeds (F), respectively. The double arrow between the two transverse lines indicates the cell layers of the OV. <b>(G, H).</b> Semi-thin transverse sections from the mid-region of mature control seeds (G) and m-seeds (H) at 30 DAP. The red rectangle in (G) and (H) indicates the position of ovular vascular trace. The arrowhead in (H) indicates the remained pericarp. <b>(I, J).</b> Higher magnification for part of the pericarp in mature control seeds (I) and m-seeds (J) at 30 DAP. It shows that the pericarp cuticular layer in (I) and persisted pericarp cells in (J). The red dots indicate the cuticular seed coat in control seeds (I) and the cells of integuments and nucellar epidermis in m-seeds are not degenerated (J). <b>(K, L).</b> Higher magnification of the OV in control seeds (K) and m-seeds (L), which showed in the red rectangle in (G) and (H). The red circle in (L) indicates the large OV in m-seeds. AL, aleurone; NP, nucellar projecton; OV, ovular vascular trace; P, pericarp; PH, phloem; PS, the pigment strand; XY, xylem; EN, endosperm. Bars = 200 µm in (A–C), 50 µm in (D–F), 500 µm in (G) and (H), 100µm in (I) and (J), and 50 µm in (K) and (L).