<i>sli-1</i> does not affect the timing of deaths or morphology of cell corpses.

<p>(A) The number and time of appearance of apoptotic cell corpses that occurred from the first cell death to the comma stage were recorded at 3-min intervals in wild-type and <i>sli-1(sy143)</i> animals using time-lapse DIC microscopy (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003115#s4" target="_blank">Materials and Methods</a>). Mean numbers of corpses at each time point were calculated from three embryos for both wild-type and <i>sli-1(sy143)</i> animals. Statistical analysis was by the Wilcoxon rank-sum test. The curves are similar (<i>p</i>>0.05). (B) The duration of cell-corpse visibility is similar in wild-type and <i>sli-1(sy143)</i> embryos. The percentage of cell corpses visible for a given period was recorded. The duration of appearance of all cell corpses recorded from three wild-type (n = 159 cell corpses) and three <i>sli-1(sy143)</i> (n = 154) embryos was analyzed. Statistical analysis was by the Wilcoxon rank-sum test. The curves are similar (p>0.5). (C) The morphology of cell corpses in wild-type and <i>sli-1(sy143)</i> embryos are similar. Arrowheads, apoptotic corpses. Embryos were at similar stages of development, approximately six minutes prior to the comma stage. Bar = 5 microns.