<i>egl-27(+)</i> promotes heat and oxidative stress survival.

<p>For A and B, day 1 adult hermaphrodite worms worms were heat stressed at 35°C for 8 hours and survival was scored at 20°C as a function of time. Additional survival data can be found in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003108#pgen.1003108.s006" target="_blank">Table S1</a>. (A) <i>egl-27(we3)</i> worms have increased sensitivity to heat-stress while <i>egl-27::GFP</i> worms are more resistant to heat stress. N2: n = 129, mean survival = 19.6 hours, median survival = 23 hours, 95% mortality = 31 hours. <i>egl-27(we3)</i>: n = 114, mean survival = 14.4 hours, median survival = 9 hours, 95% mortality = 31 hours. p = 7.8×10⁻⁸ vs. N2. Control: n = 108, mean survival = 24.6 hours, median survival = 23 hours, 95% mortality = 50 hours. <i>egl-27::GFP</i>: n = 78, mean survival = 52.4 hours, median survival = 31 hours, 95% mortality = 134 hours. p = 5.8×10⁻⁶ vs. control. (B) Loss of <i>egl-27</i> activity partially suppresses the stress resistance of <i>daf-2(e1370)</i> mutants. <i>daf-2(e1370)</i> : n = 113, mean survival = 238 hours, median survival = 296 hours. <i>daf-2(e1370)</i>; <i>egl-27(we3)</i>: n = 118, mean survival = 183 hours, median survival = 172 hours. p = 7.8×10⁻¹⁰ vs. <i>daf-2(e1370</i>). <i>daf-2(e1370)</i>; <i>daf-16(mu86)</i>: n = 128, mean survival = 19.2 hours, median survival = 23 hours, 95% mortality = 31 hours. N2: n = 129, mean survival = 19.6 hours, median survival = 23 hours, 95% mortality = 31 hours. For C and D, day 1 adult hermaphrodite worms were grown on plates supplemented with 10 mM paraquat and 30 mM FuDR and survival was scored at 20°C as a function of time. Additional survival data can be found in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003108#pgen.1003108.s006" target="_blank">Table S1</a>. (C) <i>egl-27</i> does not affect paraquat survival in wild-type and control worms. N2: n = 212, mean survival = 3.8 days, median survival = 4 days, 95% mortality = 5 days. <i>egl-27(we3)</i>: n = 243, mean survival = 3.6 days, median survival = 4 days, 95% mortality = 5 days. Control: n = 133, mean survival = 5.4 days, median survival = 5 days, 95% mortality = 9 days. <i>egl-27::GFP</i>: n = 272, mean survival = 6.1 days, median survival = 5 days, 95% mortality = 14 days. (D) <i>daf-2(e1370); egl-27(we3)</i> double mutants are less resistant to paraquat-induced oxidative stress than <i>daf-2(e1370)</i> mutants. <i>daf-2(e1370)</i>: n = 211, mean survival = 15.7 days, median survival = 14 days, 95% mortality = 29 days. <i>daf-2(e1370)</i>; <i>egl-27(we3)</i>: n = 284, mean survival = 8.2 days, median survival = 6 days, 95% mortality = 25 days (p-value vs. <i>daf-2(e1370)</i> = 0). <i>daf-2(e1370)</i>; <i>daf-16(mu86)</i>: n = 262, mean survival = 5.4 days, median = 6 days, 95% mortality = 7 days. N2: n = 212, mean survival = 3.8 days, median survival = 4 days, 95% mortality = 5 days.