COX-2 expression in tumors from 12-week-old mice housed for 9 weeks in SE compare to EE.

<p>(A) Tumor excised from EE and SE mice, 21 days after the injection were cut and labeled by indirect immunofluorescence staining for COX-2 (green) and with DAPI as nuclear counterstain (blue). (B) Immunolabeled cells for COX-2 into the tumors were quantified using ImageJ software where the SE levels were set at 100% (n = 3 per group). (C) Tumor lysates were analyzed with western-blotting with the indicated antibodies. Photographs of chemiluminescent detection of the blot are shown. The relative abundance of each band to its own β-actin was quantified using ImageJ software, and the SE levels were set at 100%. (D) Tumors excised from SE and EE mice were cut and labeled by indirect immunofluorescence staining for COX-2 (green), leptin (red) and with DAPI as nuclear counterstain (blue). Right panels show overlays of the left and middle panels where the yellow shows co-localization of COX-2 and leptin. Right panels show boxed regions at high magnification. Scale bars: 100 µm. Data presented are the mean ± SEM; * = p<0.05, NS = not significant.