<p>Representative flow cytometry data are shown. (A) The percentage of CD45RA<sup>−</sup>CD45RO<sup>+</sup>IFN-γ<sup>+</sup> cells in the CD4<sup>+</sup> T cell population (B) The percentage of CD45RA<sup>−</sup>CD45RO<sup>+</sup>IL-4<sup>+</sup> cells in the CD4<sup>+</sup> T cell population (C) The percentage of CD45RA<sup>−</sup>CD45RO<sup>+</sup>IL-17<sup>+</sup> cells in the CD4<sup>+</sup> T cell population. T cell maturation was not influenced by ustekinumab treatment.</p
<p>(A) Overlay of IFN-γ<sup><b>+</b></sup> (grey) and IL-17<sup><b>+</b></sup> (black), IFN-γ<sup><b...
<p>(A) IL-17 and IFN-γ production of CD4<sup><b>+</b></sup> T cells stimulated with different amount...
<p>A) Naïve CD4<sup>+</sup> T cells were cultured under Th17-promoting conditions with or without IL...
<p>Flow cytometric analysis showed abundant cytokine production from CD45RA<sup>−</sup>CD45RO<sup>+<...
<p>PBMCs were stained with anti-CD4 PE-cy7, anti-CD25 APC, anti-IFN-γ FITC, anti-IL-17 PE, anti-IL-4...
<p>OT-II lines were generated by repeated weekly stimulation in the presence of cytokine that polari...
<p>A. CD4+CD25- T cells were sorted by flow cytometry into CD45RO- and CD45RO+ subsets. Memory CD4+C...
<p>CD4<sup>+</sup> T cells were cultured with immature or mature BMDC at a 2/1 CD4<sup>+</sup>/BMDC ...
<p>The dot plots show representative data from healthy control subjects, illustrating the analysis m...
<p>PBMCs were isolated from individual participants and stimulated with PMA and ionomycin for six ho...
<p>(A–E) FACS-sorted naive CD4<sup>+</sup>CD62L<sup>hi</sup>CD44<sup>lo</sup>CD25<sup>−</sup>CXCR3<s...
<p>Percentage of PD-1 expressing T cells during their differentiation toward (A) Th1 or (B) Th17 lin...
<p><b>(A)</b> PBMCs were stained with anti-CD4 PE-cy7, anti-CD45RA–FITC, anti-CCR7 APC and anti-IL-1...
<p>CD4<sup>+</sup> T cells (B) were gated from lymphocytes (A) for further phenotyping. (C, D, E) Re...
<p>Total naïve CD4+ cells were isolated by homogenization and subsequent magnetic associated cells s...
<p>(A) Overlay of IFN-γ<sup><b>+</b></sup> (grey) and IL-17<sup><b>+</b></sup> (black), IFN-γ<sup><b...
<p>(A) IL-17 and IFN-γ production of CD4<sup><b>+</b></sup> T cells stimulated with different amount...
<p>A) Naïve CD4<sup>+</sup> T cells were cultured under Th17-promoting conditions with or without IL...
<p>Flow cytometric analysis showed abundant cytokine production from CD45RA<sup>−</sup>CD45RO<sup>+<...
<p>PBMCs were stained with anti-CD4 PE-cy7, anti-CD25 APC, anti-IFN-γ FITC, anti-IL-17 PE, anti-IL-4...
<p>OT-II lines were generated by repeated weekly stimulation in the presence of cytokine that polari...
<p>A. CD4+CD25- T cells were sorted by flow cytometry into CD45RO- and CD45RO+ subsets. Memory CD4+C...
<p>CD4<sup>+</sup> T cells were cultured with immature or mature BMDC at a 2/1 CD4<sup>+</sup>/BMDC ...
<p>The dot plots show representative data from healthy control subjects, illustrating the analysis m...
<p>PBMCs were isolated from individual participants and stimulated with PMA and ionomycin for six ho...
<p>(A–E) FACS-sorted naive CD4<sup>+</sup>CD62L<sup>hi</sup>CD44<sup>lo</sup>CD25<sup>−</sup>CXCR3<s...
<p>Percentage of PD-1 expressing T cells during their differentiation toward (A) Th1 or (B) Th17 lin...
<p><b>(A)</b> PBMCs were stained with anti-CD4 PE-cy7, anti-CD45RA–FITC, anti-CCR7 APC and anti-IL-1...
<p>CD4<sup>+</sup> T cells (B) were gated from lymphocytes (A) for further phenotyping. (C, D, E) Re...
<p>Total naïve CD4+ cells were isolated by homogenization and subsequent magnetic associated cells s...
<p>(A) Overlay of IFN-γ<sup><b>+</b></sup> (grey) and IL-17<sup><b>+</b></sup> (black), IFN-γ<sup><b...
<p>(A) IL-17 and IFN-γ production of CD4<sup><b>+</b></sup> T cells stimulated with different amount...
<p>A) Naïve CD4<sup>+</sup> T cells were cultured under Th17-promoting conditions with or without IL...