<p>Cells were treated with freshly isolated SPPs, DPI-treated SPPs, and heat-inactivated SPPs, individually and in combination with NADPH for 24 h. Expression of HLA-DQ and co-stimulatory molecules was analyzed by means of flow cytometry. Data are presented as means ± SEM of four independent experiments. * <i>p</i><0.05; ** <i>p</i><0.01; *** <i>p</i><0.001 vs untreated DCs, <sup># </sup><i>p</i><0.05; <sup>## </sup><i>p</i><0.01. (DPI: diphenyleneiodonium; SPP: subpollen particle; SPP<sup>H</sup>: heat-inactivated SPP; SPP<sup>SUP</sup>: supernatant of SPPs).</p
<p>(A) Human MDDCs were stimulated for 24 h with LPS, MPL, or dLOS, and expression of HLA-DR, CD80, ...
<p>After 24 h of stimulation, M-hMPV-treated moDCs were labeled by DiOC6(3) and propidium iodide pri...
(A) The relative geometric mean fluorescence intensities of CD83, CD80, CD86, and HLA-DR on MoDCs we...
<p>ELISA was used to determine the release of cytokines (IL-6, TNF-α, IL-10) and chemokine (IL-8) fr...
<p>CFSE-labeled naïve CD4<sup>+</sup> T cells were co-cultured with allogeneic moDCs pre-treated wit...
<p>Cells were incubated with freshly isolated ragweed SPPs for 1 h both in the presence and absence ...
<p>(A) Investigation of the percentage of human moDCs having attached/internalized SPPs by means of ...
<p>Human MoDCs were generated from PBMCs and treated with PBS (control) or AC hmwPSs (5 or 10 μg/mL)...
<p>(<b>A</b>, <b>B</b>) moDCs were treated with LPS and analyzed by flow cytometry. FACS histogram p...
<p>Freshly isolated CD3<sup>+</sup> pan-T cells obtained from three ragweed allergic individuals and...
<p>MoDCs were inoculated with PA in a 2:1 molar ratio with BSA for (A) 12 hrs, (B) 24 hrs, or (C) 48...
<p>moDCs were treated with M-hMPV protein 0.172 nM (gray filled) and 0.0172 nM (green line), LPS 10 ...
<p>moDCs were stimulated with bacterial pathogens or <i>E. coli</i> LPS for 24 hours. The cells were...
<p>moDCs at day 7 in culture were used for cell phagocytosis and cell differentiation status analysi...
<p>MoDCs were derived from human peripheral blood monocytes. Expression of HLA-DR, CD11c, CD14, CD16...
<p>(A) Human MDDCs were stimulated for 24 h with LPS, MPL, or dLOS, and expression of HLA-DR, CD80, ...
<p>After 24 h of stimulation, M-hMPV-treated moDCs were labeled by DiOC6(3) and propidium iodide pri...
(A) The relative geometric mean fluorescence intensities of CD83, CD80, CD86, and HLA-DR on MoDCs we...
<p>ELISA was used to determine the release of cytokines (IL-6, TNF-α, IL-10) and chemokine (IL-8) fr...
<p>CFSE-labeled naïve CD4<sup>+</sup> T cells were co-cultured with allogeneic moDCs pre-treated wit...
<p>Cells were incubated with freshly isolated ragweed SPPs for 1 h both in the presence and absence ...
<p>(A) Investigation of the percentage of human moDCs having attached/internalized SPPs by means of ...
<p>Human MoDCs were generated from PBMCs and treated with PBS (control) or AC hmwPSs (5 or 10 μg/mL)...
<p>(<b>A</b>, <b>B</b>) moDCs were treated with LPS and analyzed by flow cytometry. FACS histogram p...
<p>Freshly isolated CD3<sup>+</sup> pan-T cells obtained from three ragweed allergic individuals and...
<p>MoDCs were inoculated with PA in a 2:1 molar ratio with BSA for (A) 12 hrs, (B) 24 hrs, or (C) 48...
<p>moDCs were treated with M-hMPV protein 0.172 nM (gray filled) and 0.0172 nM (green line), LPS 10 ...
<p>moDCs were stimulated with bacterial pathogens or <i>E. coli</i> LPS for 24 hours. The cells were...
<p>moDCs at day 7 in culture were used for cell phagocytosis and cell differentiation status analysi...
<p>MoDCs were derived from human peripheral blood monocytes. Expression of HLA-DR, CD11c, CD14, CD16...
<p>(A) Human MDDCs were stimulated for 24 h with LPS, MPL, or dLOS, and expression of HLA-DR, CD80, ...
<p>After 24 h of stimulation, M-hMPV-treated moDCs were labeled by DiOC6(3) and propidium iodide pri...
(A) The relative geometric mean fluorescence intensities of CD83, CD80, CD86, and HLA-DR on MoDCs we...