Endogenous TAp63γ protein levels increase at early stages of neural differentiation.

<p>Undifferentiated (0 h) and differentiated mouse NSCs were collected for Western blot analysis or fixed for immunostaining at indicated time-points, as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052417#s2" target="_blank">Materials and Methods</a>. (A) Protein levels of TAp63γ, JMJD3, β-III tubulin, and H3K27me3 throughout NSC differentiation. Representative immunoblots (top), and TAp63γ and JMJD3 protein levels (bottom). β-Actin and H3 were used as loading controls. Immunoblots are representative of at least three different experiments. TAp63γ and JMJD3 protein levels were normalized to endogenous β-Actin protein levels and expressed as mean ± SEM for at least three different experiments. *<i>p</i><0.01 from control, undifferentiated. (B) Fluorescence staining of TAp63γ and JMJD3 throughout NSC differentiation. Hoechst dye was used for nuclear staining. Images are representative of at least 3 different experiments. Scale bar = 10 µm.