<p>ELISA was used to determine the release of cytokines (IL-6, TNF-α, IL-10) and chemokine (IL-8) from human moDCs in response to freshly isolated SPPs, DPI-treated SPPs, and heat-inactivated SPPs, in the presence and absence of NADPH, after 24 h incubation. Data are presented as means ± SEM of four individual experiments. * <i>p</i><0.05; ** <i>p</i><0.01 vs untreated DCs, <sup># </sup><i>p</i><0.05. (DPI: diphenyleneiodonium; SPP: subpollen particle; SPP<sup>H</sup>: heat-inactivated SPP).</p
<p>(A) Supernatants of cells treated for 24 h with elution buffer, LPS 10 ng/mL (positive control) a...
<p><b>A</b> 1×10<sup>5</sup> THP-1 cells, primary human monocytes, monocyte-derived DCs (moDCs), or ...
<p>Six-day cultured M-CSF MΦ were activated with IFNγ or with IL-4 to obtain M1 and M2 MΦ, respectiv...
<p>Cells were treated with freshly isolated SPPs, DPI-treated SPPs, and heat-inactivated SPPs, indiv...
<p>CFSE-labeled naïve CD4<sup>+</sup> T cells were co-cultured with allogeneic moDCs pre-treated wit...
<p>Cells were incubated with freshly isolated ragweed SPPs for 1 h both in the presence and absence ...
<p>Freshly isolated CD3<sup>+</sup> pan-T cells obtained from three ragweed allergic individuals and...
<p>Human MoDCs were generated from PBMCs and treated with PBS (control) or AC hmwPSs (5 or 10 μg/mL)...
(A) The relative geometric mean fluorescence intensities of CD83, CD80, CD86, and HLA-DR on MoDCs we...
<p>After 12 h exposure to ManLAM and/or PIM (at the concentration of 10 µg/ml and 5 µg/ml, respectiv...
<p>Human moDCs were generated in the presence of GM-CSF and IL-4. 100 nM SFA or drug vehicle were ad...
<p>Cytokine profiles of monocyte-derived DCs <i>(A)</i> and primary DCs <i>(B)</i> stimulated for 24...
<p>DCs were incubated with SL-CoV BVLPs or SARS CoV VLPs at a concentration of 10 µg/ml. DCs treated...
<p>(A) Investigation of the percentage of human moDCs having attached/internalized SPPs by means of ...
<p>The concentrations of cytokines/chemokines including IP-10 (A), RANTES (B), MCP-1 (C), IL-6 (D), ...
<p>(A) Supernatants of cells treated for 24 h with elution buffer, LPS 10 ng/mL (positive control) a...
<p><b>A</b> 1×10<sup>5</sup> THP-1 cells, primary human monocytes, monocyte-derived DCs (moDCs), or ...
<p>Six-day cultured M-CSF MΦ were activated with IFNγ or with IL-4 to obtain M1 and M2 MΦ, respectiv...
<p>Cells were treated with freshly isolated SPPs, DPI-treated SPPs, and heat-inactivated SPPs, indiv...
<p>CFSE-labeled naïve CD4<sup>+</sup> T cells were co-cultured with allogeneic moDCs pre-treated wit...
<p>Cells were incubated with freshly isolated ragweed SPPs for 1 h both in the presence and absence ...
<p>Freshly isolated CD3<sup>+</sup> pan-T cells obtained from three ragweed allergic individuals and...
<p>Human MoDCs were generated from PBMCs and treated with PBS (control) or AC hmwPSs (5 or 10 μg/mL)...
(A) The relative geometric mean fluorescence intensities of CD83, CD80, CD86, and HLA-DR on MoDCs we...
<p>After 12 h exposure to ManLAM and/or PIM (at the concentration of 10 µg/ml and 5 µg/ml, respectiv...
<p>Human moDCs were generated in the presence of GM-CSF and IL-4. 100 nM SFA or drug vehicle were ad...
<p>Cytokine profiles of monocyte-derived DCs <i>(A)</i> and primary DCs <i>(B)</i> stimulated for 24...
<p>DCs were incubated with SL-CoV BVLPs or SARS CoV VLPs at a concentration of 10 µg/ml. DCs treated...
<p>(A) Investigation of the percentage of human moDCs having attached/internalized SPPs by means of ...
<p>The concentrations of cytokines/chemokines including IP-10 (A), RANTES (B), MCP-1 (C), IL-6 (D), ...
<p>(A) Supernatants of cells treated for 24 h with elution buffer, LPS 10 ng/mL (positive control) a...
<p><b>A</b> 1×10<sup>5</sup> THP-1 cells, primary human monocytes, monocyte-derived DCs (moDCs), or ...
<p>Six-day cultured M-CSF MΦ were activated with IFNγ or with IL-4 to obtain M1 and M2 MΦ, respectiv...