TAp63γ and JMJD3 directly associate during neural differentiation.

<p>Mouse NSCs undergoing differentiation were collected and processed for immunoprecipitation assays with either p63 antibody or mouse IgG as control, as described in Material and Methods. (A) Representative immunoblot of JMJD3 and TAp63γ (top) and histogram of TAp63γ/JMJD3 endogenous association (bottom). (B) Representative immunoblot of JMJD3 and TAp63γ (top) and histogram of TAp63γ/JMJD3 association (bottom) in cells transfected with JMJD3 or JMJD3 mutant (JMJD3mut) overexpression plasmids, or no plasmid (mock). (C) Representative immunoblot of methylated lysine (MeK) and TAp63γ (top), and histogram of TAp63γ methylated levels (bottom). All densitometry values for JMJD3 and methylated lysines were normalized to the respective p63 expression, and the results expressed as mean ± SEM for at least three different experiments. §<i>p</i><0.05 and *<i>p</i><0.01 from controls. IP, Immunoprecipitation; WB, Western blot; MeK, Methyl K pan.