EPI binding hinders DNA-protein interaction (A): FTIR difference spectra of DNA in the absence and presence of increasing concentration of EPI. Curve a-d corresponds DNA (3.0 mM)+drug (a) 0 µM; (b) 3 µM; (c) 30 µM and (d) 300 µM.

<p>(<b>B</b>): Molecular modeling of DNA binding with EPI. (C): Gel retardation assay of inhibition of binding by octamer proteins with increasing concentration of EPI. The octamer binding probe (H2B gene) was exposed to 0.1–5.0 µM of EPI for an incubation period of 12 h. Control samples were incubated in the absence of drug in the reaction buffer. The reacted probe was exposed to nuclear extract from HEK293 cells. Electrophoretically retarded bands denote protein-DNA complexes due to Oct proteins present in the nuclear extract.